منابع مشابه
Solubilization pdf
PURPOSE: Micellar solubilization is a powerful alternative for. Done regarding solubilization of drugs by micelles is presented. Abstract: In this paper, the use of surfactants for solubilization of. solubilization techniques pdf Polyelectrolytes, pH, and temperature on dye solubilization has been.Solution chemistry, solubility and solubilization. Dissolution limited bioavailability is a very c...
متن کاملSolubilization of Peptides
ity. The amino acid composition will provide a fi rst indication: before choosing a solvent, the sequence should be studied and the number of acidic, basic and neutral residues should be determined. The side-chain functionalities of acidic and basic amino acids will be charged at physiological pH. The nature of the Nand the C-terminal functionality has to be taken into consideration. The neutra...
متن کاملSolubilization and properties of galactosyltransferase and sulfotransferase activities of Golgi membranes in Triton X-100.
UDP-galactose-A-acetylglucosamine galactosyltransferase of rat liver Golgi apparatus is membrane-bound, that is, it is not released by treatment with salt or disruption of the Golgi saccules using N, decompression in a Parr bomb. The enzyme can be solubilized to greater than 90% by treatment of rat liver Golgi membranes with 2 mg of Triton X-lOO/ml. We have determined the sedimentation constant...
متن کاملSolubilization of proteins from bovine brain coated vesicles by protein perturbants and Triton X-100
To identify integral and peripheral membrane proteins, highly purified coated vesicles from bovine brain were exposed to solutions of various pH, ionic strength, and concentrations of the nonionic detergent Triton X-100. At pH 10.0 or above most major proteins were liberated, but four minor polypeptides sedimented with the vesicles. From quantitative analysis of phospholipids in the pellet and ...
متن کاملDifferential solubilization of inner plasma membrane leaflet components by Lubrol WX and Triton X-100.
A commonly-used method for analysing raft membrane domains is based on their resistance to extraction by non-ionic detergents at 4 degrees C. However, the selectivity of different detergents in defining raft membrane domains has been questioned. We have compared the lipid composition of detergent-resistant membranes (DRMs) obtained after Triton X-100 or Lubrol WX extraction in MDCK cells in ord...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1952
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.72.3_338